System Manufacturer/Model Number Built by Myself OS Windows 7 Ultimate SP1 (64-bit) CPU Intel Core i5-2310 @ 2.90GHz Motherboard MSI PH67A-C43 (B3) Memory Kingston KVR 16GB (4 x 4GB) DDR3 @ 1333MHz Graphics Card PNY XLR8 GTX 560 Ti 1GB GDDR5 Sound Card Realtek HD Audio (ALC 892) Monitor(s) Displays LG W2353V-PF Screen Resolution 1920 x 1080 (HDMI)
Keyboard Logitech MK320 Wireless Keyboard Mouse Logitech MK320 Wireless Mouse PSU Thermaltake Toughpower XT 575w Modular 80 PLUS Bronze Case Cooler Master CM Storm Enforcer Cooling Thermaltake Frío, CM MegaFlow 200mm (2), CM 120mm (1) Hard Drives Samsung HD502HJ (500GB), ExcelStor J8080S (80GB) Internet Speed dl: 1024kbps, ul: 512kbps
No it won't...Prefetch files are an index not the actual executable files themselves. There is no reason to clear it. Prefetch and SuperFetch do not store copies any where. If the files a virus uses are gone, they are gone.
The simple answer is yes, you can purge prefetch without issues.
There is no real reason other than clearing out old data, no other reason.
If you have a cleanup tool that does it, there i no reason to tell it not to clean it.