Not if you transfer Windows 7 via an image. SSD - Install and transfer the Operating System
If you reinstall you'll need to move via the Location tabs again.
However as you should have discovered by now, there are problems with moving the User profile or folders to another HD such as the HD then becomes part of the System and cannot be separated for imaging purposes, some download .exes will give unfixable permissioning errors, etc.
For these and other reasons if you reinstall we now suggest you instead simply rightclick on each user folder on the HD to add it to the related Library - Include a Folder - Windows 7 Forums -
you'll need to create one for Downloads. Leave the empty C User folder in Library so you can easily see if anything writes there to drag it over to HD folder.
If you're only reimaging and have had no problems with this so far then you can leave it the same.