Win7 No Longer Boots After Removing Spare Drive, Why?

Hello guys,

I have two WD black 1 TB drives in a raid 0 array, this is what I have Win7 installed on, I also have a 1 TB Seagate drive that I was using to backup windows to, but now I'm backing up to a NAS, the Seagate drive is just setting in my system not doing much so I want to remove it and put it in to another system.

The problem is, if I remove the Seagate drive windows no longer boots I get the following error "Reboot and select a proper boot device or insert boot media in selected boot device and press any key", I have checked my boot priority in my bios and my CD drive is first then the raid array. I have also checked to make sure that the Seagate drive was not a part of the raid array, it was not just the two WD drives. If I reattach the Seagate drive windows boots without any problem, it does not appear that there is any data saved on the Seagate drive. If I go in to the windows disk manager the C: drive has 1863GB so two 1 TB drives and my I: drive the Seagate has 931GB and is showing 14GB used. The C: status is as follows (Healthy, Boot, Page File, Crash Dump, Primary Partition) my I: drive status is as follows (Healthy, System, Active, Primary Partition)

I'm not sure what to try next.

Thanks so much Golden, any help at this point would be great

Thanks so much for the fast reply guys, so I'm guessing this is not a simple fix?

Thanks so much guys, I will give that a shot, keep ya all posted.