Boot Error using PNY USB 16 GB

[Pushyfurball]

I have been trying this for some time now, but I cannot get my computer to boot from my USB drive. I have used "Bootable USB Drive Creator Tool" to make it FAT32 with the specific files I need on there. My main goal as of right now is to get my PC to boot from USB and have no other HDD in there besides the one I want to use to format for my Xbox 360 and use an the add-on instead of purchasing a new 250 GB HDD. It is a WD2500BEVS... I have tried using different ports and was open to a suggestion of different software to try or something else. When I restart my PC, I press F2 which is my boot option and click USB and in my BIOS it is set to start from USB>CD>HDD.

Again, I am open to ALL suggestions at this time.

Thanks in advanced.

---EDIT---
Also, the only thing on my USB is the following: hddhackr.exe , HDDSS.BIN , ReadMe.txt
---END EDIT---

 

[jumanji]

What exactly are you trying to do?

You want to boot and run Windows from a USB pendrive and then remove/use the system drive for your Xbox??

Nope, it is not possible. Run your Windows as it should.